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After mixing with bacteriostatic water, many research guides use 3-4 weeks as the practical multi-use window under refrigeration

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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

Nat Genet 10(1):111113 Ganguly P, Alam SF (2015) Role of homocysteine in the development of cardiovascular disease